Confocal Laser Scanning Microscope

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The Nikon C1Si CLSM is build on a Nikon eclipse Ti microscope stand equipped with 4 lasers resulting in the laser lines: •405 nm •457 nm •488 nm •514 nm •561 nm •640nm.

The system is filter block based and has 4 detectors (PMTs) which can be used as 3 fluorescence and one transmitted light detector or in a configuration with 4 fluorescence detectors. Besides the standard PMTs this system has also a spectral detector unit with an array of 32 PMTs. This array makes for fast spectral imaging with a resolution of 1.5 nm, 5 nm or 10 nm and is the fastest microscope based spectral imager in our facility.