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FV1000 confocal laser scanning microscope

AVAILABILITY By appointment or online booking
TRAINING Training is required to use this item and we can arrange this if needed.
CONTACT 1 Dr Kees Straatman (HWB) x7085/2263
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Please visit http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif/Equipment/microscopes/olympus-fv1000-confocal-microscope

This microscope is an inverted IX81 motorized microscope equipped with a: UV laser diode (405 nm, 50 mW) Argon laser, multi-line: 457/488/515 nm (40 mW) yellow diode laser (559 nm, 20 mW) red diode laser (635 nm, 20 mW)

The selection of the laser line is controlled via an Acousto Optical Tunable Filter (AOTF). The use of an AOTF allows excitation intensity balancing within an image, flexible area bleaching e.g. for FRAP/FLIP/Photo-activation experiments and line sequential scanning to avoid cross-talk between channels (multi-tracking). For detection of fluorescence signal 3 PMT and 2 high sensitive GaAsP detectors (HSDs) are installed + a transmitted light detector. Two of the detection channels are equipped with a galvano grating spectral scanning systems for fluorescence emission detection with variable setting of wavelength band. This allows fine tuning of the emission detection and high speed spectral scanning. The spectral range of the PMT detectors is appr. 300 nm to 850 nm. The detectors can be used in two modes (1) conventional photo current detection mode (2) high sensitivity photon counting mode.

Item ID #922.

Last Updated: 6th June, 2014

FV1000 confocal laser scanning microscope